Blood Cells A Practical Guide, 5 edition by Barbara J. Bain

By Barbara J. Bain

- allows either the haematologist and laboratory scientist to spot blood cellphone beneficial properties, from the most typical to the extra obscure
- offers crucial info on equipment of assortment, blood movie instruction and marking, including the rules of handbook and automatic blood counts
- thoroughly revised and up-to-date, incorporating a lot newly released info: now contains suggestion on extra checks whilst a selected analysis is suspected
- four hundred prime quality pictures to assist with blood phone identification
- Highlights the aim and scientific relevance of haematology laboratory assessments all through

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Extra resources for Blood Cells A Practical Guide, 5 edition

Sample text

1). It should be noted that in the USA blood is usually taken into K3EDTA, and in the UK it is more usually taken into K2EDTA; because of the cell shrinkage that occurs, the microhaematocrit with K3EDTA is about 2% lower than with K2EDTA [17]. The precision of the microhaematocrit can be improved by making at least three replicate measurements and taking the average; this is necessary when a manual PCV is used to calibrate an automated instrument. It has been recommended that, because of the increase in micro‐haematocrit that occurs with deoxygenation, blood should be fully oxygenated when a microhaematocrit is used for calibration of an automated instrument [16].

Aberrant impulses can be edited out electronically. Sheathed low or hydrodynamic focusing can direct cells to the centre of the aperture to reduce the problems caused both by coincidence and by aberrant impulses. Both sheathed low and sweep low behind an aperture can prevent recirculation of cells. Impedance counters generally produce very precise measurements of cell volume and haemoglobin content and concentration. However, there are some inaccuracies inherent in the method, which are greater when cells are abnormal.

Because of the smallness of the tube, reading the level correctly can be dificult. Tubes may taper or be of an uneven bore. The seal is not lat, tending to be convex if modelling clay is used and concave if heat sealing is employed, although the error introduced by the type of seal is usually minor [12]. The amount of plasma trapping is usually around 1–3%, but is variable. 1). It should be noted that in the USA blood is usually taken into K3EDTA, and in the UK it is more usually taken into K2EDTA; because of the cell shrinkage that occurs, the microhaematocrit with K3EDTA is about 2% lower than with K2EDTA [17].

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