By A H Rebar; et al
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Extra resources for A Guide to Hematology in Dogs and Cats
Platelet count can be estimated from the blood smear. 10-12 platelets per oil immersion field (100x nose piece objective) is an appropriate number in the dog and cat. In the absence of obvious clumping, fewer than 10-12 platelets per 100x field suggests thrombocytopenia and indicates the need for a quantitative platelet count. Advantages ® Manual counting is the least expensive in terms of equipment, supplies. ® Can be performed in mobile clinics. Limitations ® Time consuming; most expensive in terms of professional staff time.
WBC cell count must be corrected for nucleated RBCs. ® The overlap in size between feline platelets and erythrocytes leads to overestimates of the erythrocyte count and underestimates platelet numbers. ® Clumping of leukocytes leads to undercounting, and artifactual leukopenia. ® Only nuclear material is analyzed not cytoplasmic. 23 TNM GuideToHema:03058 TNM text_cp 6/28/07 3:49 PM Page 24 ® Does not distinguish between bands and segmented neutrophils; consequntly a left shift cannot be determined from impedance data.
Although recent breakthroughs in technology have reduced the cost so that these units are economically justified in most hospitals, they may not be economical in small practices or those that make minimal use of hematology. ® Examination of the blood film is still needed to detect abnormalities such as the presence of left shift, toxicity, reactive lymphocytes, blast cells, mast cells, microfilaria and red cell parasites. 26 TNM GuideToHema:03058 TNM text_cp 6/28/07 3:49 PM Page 27 3 LABORATORY METHODS IN HEMATOLOGY Figure 3-14 Laser flow cytometry detects and counts individual cells in microdroplets as they pass through a laser beam.